The objective of this study was to determine whether age affects the performance of female C57BL/6J mice during a battery of behavioral tests. Female C57BL/6J mice were subjected to three consecutive behavioral tests during young adulthood (2-3.5 months) and again at middle age (10-11 months). These tests were the elevated plus maze (EPM), open field test (OFT), and tail suspension test (TST), which are commonly used to assess anxiety-like, exploratory, and depression-like behaviors in mice. Each animal was randomly assigned to one of six groups (5-6 mice/group), where the sequence of the tests within the test battery was varied for each group. The performance of the animals in each test was video recorded and a number of behavioral parameters were measured, including the time spent on the open arms of the EPM, the number of grid squares crossed in the OFT, and immobility time in the TST. Statistical analyses were performed to examine the potential effects of age and group (i.e., test sequence) on behavior during these tests. The results demonstrated that the female mice exhibited more exploratory behavior when they were younger than when they were older. Furthermore, there was evidence that behavioral types (e.g., shy vs. bold) were persistent as the animals aged. An effect of test sequence on behavior was also observed, but this effect was limited to the performance of younger mice in the EPM. These findings suggest that the age of the animals and test sequence are important considerations when designing behavioral studies of mice.