A precise and cost-effective fish flume for assessing swimming performance in fishes


SOCIETY FOR INTEGRATIVE AND COMPARATIVE BIOLOGY
2021 VIRTUAL ANNUAL MEETING (VAM)
January 3 – Febuary 28, 2021

Meeting Abstract


P40-2  Sat Jan 2  A precise and cost-effective fish flume for assessing swimming performance in fishes Phillips, QP*; Karra, P; Minicozzi, MR; Minnesota State University, Mankato qpp2@nau.edu

Zebrafish are a commonly used vertebrate model organism for issues related to genetics, ecotoxicology, and development. Being able to accurately measure swimming performance in fishes is crucial to understanding their development and physiology. Research quality fish flumes often come at high price which is not ideal for a primarily undergraduate university where research is not the focus. Because of this, we have designed and built a research quality fish flume to test novel research questions related to swimming performance. We designed our flume with two spherical-impeller pumps, connected in series, with the option to summon one or both pumps programmatically. Our flume also utilizes a modulating control valve which is needed to achieve low flow rates. The flume utilizes an Omega flowmeter capable of providing relatively high resolution. Flowrate is controlled through a closed-loop PI (proportional-integral) control scheme implemented using myRIO (National Instruments) that is transferable to any system with the same controller, even with some variation in other components of the system. Our flume can achieve flow rates between 0-40 cm/s with data recorded every 100 ms or faster, depending on flowrate. All flumes experience some degree of variance in flow when attempting a target flow rate. This is to be expected as the computer records immediate flow rate and adjusts the output of the pumps accordingly. With our flume, we achieved very precise flow rates (+/- 0.06 cm/s), limiting the amount of variance around the set rate of flow. To show that our flume works, we investigated swimming performance in zebrafish. The design and control of this flume will be kept open source such that those in a research setting are able to replicate this work and build a quality budget machine for analysis of swimming performance in fishes.

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