BUCKLEY, Bradley A.; GRACEY, Andrew Y.; SOMERO, George N.; Stanford University, Hopkins Marine Station; Stanford University, Hopkins Marine Station; Stanford University, Hopkins Marine Station: DNA microarray analysis of gene expression during heat stress in the eurythermal goby Gillichthys mirabilis

Exposure to elevated but sub-lethal temperatures can have deleterious effects at the cellular level, including abnormal protein denaturation and aggregation. The heat shock response, in which molecular chaperone proteins are rapidly produced in response to thermal stress, has been well described in numerous model and non-model species. Less well understood, is the holistic effect temperature stress can have on a genomic scale. Here, DNA microarrays were used to determine the effect of exposure to high temperature on the expression of several thousand genes in the tissues of the eurythermal goby, Gillichthys mirabilis. Both emergency heat shock gene expression and the patterns of up- and down-regulation of metabolic house-keeping genes are described.