Meeting Abstract
P3.140 Jan. 6 Transnasal Delivery of a GnRH Analog in Yucatan Minature Swine SOWER, Stacia A.; KAVANAUGH, Scott I.*; SANTOS, Mark; ROGERS, Zoe; SCHULTZ, Bernardine M.; BUSHOLD, Geoff; WELLS, Roger; GYURIK, Robert; University of New Hampshire, Durham; University of New Hampshire Durham; University of New Hampshire, Durham; University of New Hampshire, Durham; University of New Hampshire, Durham; University of New Hampshire, Durham; University of New Hampshire, Durham; Bentley Pharmaceuticals Inc., Exeter sasower@cisunix.unh.edu
The overall goal of the project is the development of topical and mucosal delivery of neuropeptide hormones. The hormone tested in these experiments is gonadotropin-releasing hormone (GnRH), the major hypothalamic hormone controlling reproduction in all vertebrates. We have completed two major studies using Yucatan miniature swine and nasal application of a special combination of a formulation with the GnRH hormone analog. The specific objective included the testing of special formulations with a GnRH analog as nasal applications to determine the quality and rate of absorption of the administered hormone analog. A specific radioimmunoassay for a mammalian GnRH hormone analog was used to measure the GnRH analog that was administered. Four experiments were performed during October 2005 and June 2006 each using four miniature swine that received four intranasal treatments, one of which was a control. Treatments were administered on Days 1 and 3 and 8 and 11. Following each treatment, blood samples were collected from the swine via a catheter at 0, 30, 60, 90, 180 min and 24 hr. Plasma was separated by centrifugation and stored at -80�C until analyzed for the GnRH analog. In summary, all treatments were successfully delivered by the intranasal route. The highest concentration of GnRH analog detected was the GnRH analog in the Bentley formulation. In conclusion, intranasal delivery of a small peptide is effective and may lead to eventual use in medical therapies. Supported by IRC Bentley Pharmaceuticals to SAS, and UNH Undergraduate Research Support to MS. sasower@cisunix.unh.edu
