Meeting Abstract
27.2 Wednesday, Jan. 5 Molecular Phylogeny of Isopoda Bopyroidea and Insights into Host/Parasite Coevolution BOYKO, C.B.*; MOSS, J.; WILLIAMS, J.D.; SHIELDS, J.D.; Dowling College; Virginia Institute of Marine Science; Hofstra University; Virginia Institute of Marine Science cboyko@amnh.org
The evolutionary history of the Bopyroidea, obligate parasites using decapods as definitive hosts, dates to the Jurassic. Bopyroidea (Bopyridae, Dajidae and Entoniscidae) are known from primitive (Dendrobranchiata: 39 bopyrids, 4 dajids; Caridea: 187 bopyrids, 4 dajids, 1 entoniscid), and derived decapod hosts (Anomura and Brachyura: 286 bopyrids, 35 entoniscids). We examined monophyly of Bopyroidea and Bopyridae and degree of congruence between these parasites and their hosts based on 18s rRNA from representatives of 8/9 bopyrid subfamilies, 2 dajids, and 1 entoniscid. PCR amplifications from genomic DNA used published primers. DNA fragment lengths were ~2,500 bp. Four overlapping sub-fragments were cloned, bi-directionally sequenced, edited, and analyzed. MacVector, R-coffee, Paup were used for analyses, and final alignment included complete sequence data for all taxa (alignment length = 3998 bp). 18s rRNA data indicate a pattern of evolution in Bopyridae different from that of other studies, with “basal” pseudionines as derived taxa. 3 subfamilies of abdominal bopyrids are not sister taxa, suggesting that abdominal parasitization occurred at least twice. Entoniscidae appear as sister taxon to the Ioninae (which is paraphyletic), and this clade is sister to the remainder of Bopyridae or sister to Dajidae+Entophiliinae. Entophiliinae were not closely related to the rest of Bopyridae, and is sister to Dajidae, suggesting that parasitism on decapods may have evolved twice and that Bopyroidea is not monoplyletic. Our data indicate that host switching occurred multiple times in Bopyridae. This is the first cladistic analyses of Bopyroidea and serves as a catalyst for further testing of co-evolutionary hypotheses between these parasites and their decapod hosts.
