Meeting Abstract

P2.18  Saturday, Jan. 5  Characterization of low density lipoprotein receptor (LDLR) gene superfamily members in decapod crustaceans JI-HYUN, L.*; HYUN-WOO, K.*; Department of Marine Biology, Pukyong National University, Busan 608-737, Republic of Korea; Department of Marine Biology, Pukyong National University, Busan 608-737, Republic of Korea ellen3235@gmail.com

As low density lipoprotein receptor (LDLR) gene superfamily members, the vitellogenin receptor (VgR) or lipophorin receptors (LpR) play an important role in oocyte development via receptor-mediated endocytosis of yolk protein precursors such as vitellogenin (Vg) or lipophorin (Lp). In the present study, we identified five cDNAs encoding the putative VgR/LpR/LDLRs from the morotoge shrimp (Pandalopsis japonica) and the chinese mitten crab (Eriocheir sinensis) by the combination of EST database analysis and tranditional PCR-based cloning strategy. First sequences (PJ-VgR) exhibited the highest similarity to the VgR from Macrobrachium rosenbergii (52%). Second PJ-LpR 1 was similar to LpR from Aedes aegypti (44%). Third PJ-LpR 2 turned out to be homolog of LpR from Tribolium castaneum (53%). Fourth PJ-LDLR exhibited highest similarity to LDLR from Aedes aegypti (57%). Finally, fifth ES-VgR appeared to be ortholog of VgR from Penaeus monodon (37%). They all exhibited conserved domain organizations as LDL receptor family members : ligand binding domain(LBD), epidermal growth factor (EGF) – like domain, YWTD domain, transmembrane domain, cytoplasmic domain. In order to understand an evolutional relationship among different VgR/LpR/LDLR family members in arthropod, a phylogenetic and structural analysis were also performed. Hepatopancreatic or gonadal expression of each gene was also determined by both qualitative and quantitative PCR strategy. The relationship between gonadosomatic index (GSI) and transcriptional level of five receptor genes was also determined.