Meeting Abstract
P1.157 Friday, Jan. 4 Comparison of the proteomic responses in adductor muscle tissue of Mytilus galloprovincialis and M. trossulus to acute heat shock MIER, J.S.*; ZUZOW, M.; TOMANEK, L.; California Polytechnic State University, San Luis Obispo; California Polytechnic State University, San Luis Obispo; California Polytechnic State University, San Luis Obispo jmier@calpoly.edu
Recent anthropogenic influence has caused increases in global temperature. The potential rises in temperature can impose thermal tolerance constraints which can alter marine ecosystems and restructure the extent of communal diversity. Organisms able to acclimate and adapt to thermal stress will be more resilient to the episodic and more extreme disturbances caused by climate change. To assess the biochemical mechanisms imposing thermal limits, we used a discovery based approach by comparing the proteomic responses of two species of the genus Mytilus (M. galloprovincialis & M. trossulus) that differ in thermal tolerance in response to acute heat stress. These mussels were acclimated to 13°C seawater for four weeks and then exposed to acute heat stress (24°C, 28°C, and 32°C respectively, with a 13°C control) for 1 h before being returned to 13°C seawater for a 24-h recovery. Multiple tissues were excised including the gill, adductor and mantle tissues. Gill tissue was then homogenized and proteins were separated using 2D gel electrophoresis followed by protein identification through MALDI TOF/TOF mass spectrometry. Protein expression profiles differed between the more heat-tolerant Mytilus (i.e., M. galloprovincialis) and the more heat-sensitive congener (i.e., M. trossulus), including changes in the abundances of proteins involved in cytoskeleton, molecular chaperoning, oxidative stress, energy metabolism and proteolysis. Currently, we are using the same proteomic approach to characterize the adductor muscle to elucidate whether similar changes are observed in the proteome of other tissues. These implications can provide insight as to the physiological mechanisms that govern the response to acute heat stress.
