Meeting Abstract
Adiponectin is a hormone secreted primarily by adipocyte, and its functions associated with obesity and reproduction are mediated by adiponectin receptors. Since these adiponectin receptors genes have already been identified by genetic study of crustaceans, we isolated and characterized adiponectin receptor (Pot-AdR) cDNA from swimming crab, Portunus trituberculatus with degenerated PCR and RACE analysis The cloned Pot-AdR cDNA was consisted of a 1549 nucleotide sequence, and ORF sequence (1,236bp) which encoded a protein of 411 amino acids. Pot-AdR exhibited the highest amino acid sequence identity (95.6%) to amino acid sequence corresponding to transcriptome (GenBank accession number: KA662395) known from the Chinese mitten crab, Eriocheir sinensis. In addition, transcriptional variant of Pot-AdR was identified by 3’RACE experiment, and the rest sequence of 3’ region is on confirmation by analysis. End-point RT-PCR results showed that Pot-AdR gene was ubiquitously expressed in almost all sexes tissues except the epidermis and eyestalk of male, and expressed highly in the muscle tissues and hepatopancreas. To determine Pot-AdR function, As the synthetic Pot-AdR dsRNA (30uM – 50uM) were treated by injection through the arthrodial membrane of the last walking leg of crab, Pot-AdR functions of muscles and hepatopancreas is under through RT-PCR analysis of all tissues. It suggest that adiponectin function maybe is related to physiological conditions and various function.
