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Meeting Abstract
The developmental environment plays a pivotal role in shaping phenotypes and fitness of all organisms. Perhaps the most enigmatic example of environmental effects is the influence of developmental temperature on an individual’s sex, a phenomenon known as temperature-dependent sex determination (TSD). The first description of TSD was based on a study conducted 50 years on an African lizard (Agama agama). Although novel at this time of publication, this landmark study consisted of low sample sizes and provided a poor description of the sex-determining reaction norm in this species. Our goal was to revisit this work and better characterize the pattern of TSD in A. agama. In addition, we aimed to quantify the effects of constant and fluctuating incubation temperatures on a variety of fitness-relevant traits of offspring. Eggs were obtained from an invasive population of A. agama in Miami, FL, and randomly assigned to one of nine incubation treatments: six constant temperature treatments and three fluctuating treatments that mimic field conditions. We then measured hatchling morphology (snout-vent length, head size, mass), growth, and sprint performance as indicators of fitness. Size measurements will be continuously taken every six weeks to determine the ontogenetic timing of sexual dimorphism and to determine if sexual dimorphism is influenced by incubation temperature. Preliminary data suggest that warm incubation temperatures produce mostly female offspring. This ongoing research will provide a critical evaluation of the long-term effects of developmental temperature on fitness-relevant traits, and provide insights into the adaptive significance of TSD.