Meeting Abstract

32.6  Monday, Jan. 5 09:15  Cellular metabolic rates in cultured primary dermal fibroblasts and myoblast cells from fast-growing and control Coturnix quail JIMENEZ, A.G.; COOPER-MULLIN, C. *; ANTHONY, N.B. ; WILLIAMS, J.B.; The Ohio State University; The Ohio State University; University of Arkansas; The Ohio State University ccooper-mullin@my.uri.edu

Although fibroblast cells have been used in pivotal experiments in medicine, physiology, physiological-ecology and conservation biology, the link between the physiology of fibroblasts and the physiology of other cell types of the same animal is unknown. Dermal fibroblasts play an important role in cutaneous wound repair, and in generating new connective tissues, but were thought to be metabolically inactive until needed to mend tissue damage. Therefore, the question remains, are fibroblasts a good representative model system for the metabolic profile of the whole organism, as compared with cells isolated from other tissues? To determine whether fibroblasts have the same cellular metabolic profile as myoblasts cells isolated from muscle, we cultured myoblasts and fibroblasts from two lines of Japanese quail (Coturnix coturnix japonica), one that had been artificially selected for fast growth for over 60 generations and a control line. The lines were paired in each individual quail. Isolated primary fibroblasts and myoblasts from the fast-growth line had higher rates of oxygen consumption, glycolysis and higher mitochondrial volume than cells isolated from the control line. These findings indicate that fibroblasts are a representative system on the cellular level for a whole organism metabolic signature. Additionally, fibroblasts had higher rates of metabolism for every parameter measured, including rates of oxygen consumption, glycolysis and mitochondrial volume, than myoblast cells isolated from the same individual.